84-2337-63　［取扱停止］GADD 45, gamma (CR6, Cytokine responsive Protein CR6, Cytokine-responsive Protein CR6, DDIT-2, DDIT2, DNA Damage-inducible transcript 2 Protein, GA45G, Gadd45g, Growth arrest and DNA Damage inducible gamma, Growth arrest and DNA Damage-inducible Protein GADD45 gamma, GRP17) 50ul　222845

特徴

• Cell cycle progression is subject to arrest at G1 and G2 checkpoints in response to DNA damage, presumably to allow time for DNA repair prior to entry into S and M phase, respectively.
• The p53 tumor suppressor is required for one such G1 checkpoint and functions to upregulate expression of GADD 45 and p21.
• GADD 45 binds both Cdks and PCNA, a protein involved in DNA replication and repair.
• GADD 45 stimulates DNA excision repair in vitro and inhibits entry of cells into S phase.
• Thus, it has been suggested that GADD 45 may serve as a link between the p53-dependent cell cycle checkpoint and DNA repair.
• GADD 45-like proteins, GADD 45beta and GADD 45γ, have been shown to be induced by environmental stresses.
• GADD 45beta and GADD 45γ are thought to induce p38/JNK activation via MEKK4 activation.
• Applications:Suitable for use in Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.