Critical components of this process include adapter protein such as Shc, IRS-1 and Gab 1 (GRB-associated binder-1) that lack detectable catalytic activity.
These are immediate substrates of receptor tyrosine kinase activity and serve to link activated receptors to downstream signaling components.
Whereas Shc has been implicated in signaling by diverse receptor families, IRS-1 serves primarily as the major Insulin receptor substrate.
Shc and Gab 1 also participate in Insulin signaling by linking the Insulin receptor to Ras by forming complexes with GRB2 (another adapter protein) and Sos independently of IRS-1.
The Gap 1 related protein, Gab 2, associates with SH2 domain-containing proteins, such as SHP2, and it is involved in a novel pathway for cytokine-induced gene activation.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of Gab 2. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 587-634 of Human Gab 2.
