Fyn is a membrane-associated, non-receptor protein tyrosine kinase ~59kDa, which belongs to the Src family of cytoplasmic tyrosine kinases.
Fyn is very strongly similar to mouse Fyn, v-yes and c-src.
Fyn is expressed predominately in tissues of neuronal and hematopoietic origin.
Neuronal Fyn and hematopoietic Fyn differ at the junction of the SH2 and kinase domains due to tissue specific alternative splicing.
Fyn has been shown to be involved in B cell and T cell activation as well as keratinocyte differentiation.
In T cells, Fyn associates with the T-cell antigen receptor and Thy1.
Applications:Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Immunoprecipitation: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of Fyn, phosphorylated Y530. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IHC IP WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic phosphopeptide derived from human Fyn around the phosphorylation site of Tyrosine 530.
