The frizzled gene, originally identified in Drosophila melanogaster, is involved in the development of tissue polarity.
The mammalian homolog of frizzled as well as several secreted mammalian frizzled-related proteins (FRPs) have been described.
The frizzled proteins contain seven transmembrane domains, a cysteine-rich domain in the extracellular region and a carboxy terminal Ser/Thr-xxx-Val motif.
They function as receptors for Wnt and are generally coupled to G proteins.
The frizzled-9 gene is located within the Williams Syndrome common deleted region at chromosomal band 7q11.23.
Heterozygous deletion of the frizzled-9 gene may contribute to the Williams Syndrome phenotype.
In mouse, frizzled-9 overexpression can induce the hyperphosphorylation and relocalization of Dvl-1 from the cytoplasm to the cell membrane and cytosolic beta-catenin accumulation.
In rat, frizzled-9 is important in Wnt/beta-catenin signaling in 293T cells.
Frizzled-9 is expressed predominantly in brain, testis, eye, skeletal muscle, and kidney.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of Frizzled-9. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 571-620 of Human Frizzled-9.
