The frizzled gene, originally identified in Drosophila melanogaster, is involved in the development of tissue polarity.
The mammalian homolog of frizzled, as well as several secreted mammalian frizzled-related proteins: FRP-1 (also designated SARP2), FRP-2 (also designated SARP1), FRP-3, FRP-4 and SARP3 (also designated FRP-5), have been identified.
The frizzled proteins contain seven transmembrane domains, a cysteine-rich domain in the extracellular region and a carboxy terminal Ser/Thr-xxx-Val motif.
They function as receptors for Wnt.
frizzled-7 has a Lys-Thr-X-X-X-Trp motif which is involved in the activation of the Wnt/beta-catenin signaling pathway.
frizzled-7 is an integral membrane protein showing a high expression in adult skeletal muscle, fetal kidney, fetal lung, adult heart, brain and placenta.
It is specifically expressed in squamous cell esophageal carcinomas.
Applications:Suitable for use in Immunofluorescence and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of human Frizzled-7. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：IF WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to amino acids 61-110 from human Frizzled-7.
