The frizzled gene, originally identified in Drosophila melanogaster, is involved in the development of tissue polarity.
The mammalian homolog of frizzled, as well as several secreted mammalian frizzled-related proteins: FRP-1 (also designated SARP2), FRP-2 (also designated SARP1), FRP-3, FRP-4 and SARP3 (also designated FRP-5), have been identified.
The frizzled proteins contain seven transmembrane domains, a cysteine-rich domain in the extracellular region and a carboxy terminal Ser/Thr-xxx-Val motif.
They function as receptors for Wnt.
The frizzled-1 gene maps to human chromosome 7q21 and is expressed in adult heart, placenta, lung, kidney, pancreas, prostate and ovary and in fetal lung and kidney.
Frizzled-2 is expressed in adult heart and fetal brain, lung and kidney.
The frizzled related proteins FRP-1, FRP-2, FRP-3, FRP-4 and SARP3 are secreted proteins of ~30kDa that contain regions of homology to the cysteine-rich ligand-binding domain of frizzled and a conserved hydrophilic carboxy terminal.
Applications:Suitable for use in Immunohistochemistry and Immunofluorescence.
Other applications not tested.
Recommended Dilution:Immunohistochemistry: 1:50-1:200Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, pH 7.2, 0.02% sodium azide, 50% glycerol
Specificity：Recognizes endogenous levels of human Frizzled-5/8. Species Crossreactivity: mouse, rat
Isotype：IgG
Calc Applications Abbrev：IF IHC
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 42-90 of Human Frizzled-5.
