The frizzled gene, originally identified in Drosophila melanogaster, is involved in the development of tissue polarity.
The frizzled proteins contain seven transmembrane domains, a cysteine-rich domain in the extracellular region and a carboxy-terminal Ser/Thr-XXX-Val motif.
The proteins in this family function as receptors for Wnt and are generally coupled to G proteins.
Frizzleds are members of the G protein-coupled receptor superfamily.
Frizzled-2 is expressed in the fetal kidney and lung and in the adult ovary and colon.
Frizzled-2 mediates the Wnt/cGMP/Ca2+ pathway.
It binds Wnt proteins and signals by activating the release of stored calcium.
Frizzled-2 expression is regulated by Angiotensin II.
Activated frizzled-2 suppresses the activity of protein kinase G, and activates NFAT-dependent transcription, the phosphatidylinositol pathway and calcium sensitive enzymes.
Applications:Suitable for use in Immunofluorescence, Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of Frizzled-2. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IF WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 208-260 of Human Frizzled-2.
