84-2336-27　［取扱停止］Frizzled-1 (Frizzled-1, Frizzled1, Frizzled 1, Fz-1, hFz1, FzE1, FZD1) 100ul　222777

特徴

• The frizzled gene, originally identified in Drosophila melanogaster, is involved in the development of tissue polarity.
• The mammalian homolog of frizzled, as well as several secreted mammalian frizzled-related proteins: FRP-1 (also designated SARP2), FRP-2 (also designated SARP1), FRP-3, FRP-4 and SARP3 (also designated FRP-5), have been identified.
• The frizzled proteins contain seven transmembrane domains, a cysteine-rich domain in the extracellular region and a carboxy terminal Ser/Thr-xxx-Val motif.
• They function as receptors for Wnt.
• The frizzled-1 gene maps to human chromosome 7q21 and is expressed in adult heart, placenta, lung, kidney, pancreas, prostate and ovary and in fetal lung and kidney.
• Frizzled-2 is expressed in adult heart and fetal brain, lung and kidney.
• The frizzled related proteins FRP-1, FRP-2, FRP-3, FRP-4 and SARP3 are secreted proteins of ~30kDa that contain regions of homology to the cysteine-rich ligand-binding domain of frizzled and a conserved hydrophilic carboxy terminal.
• The gene encoding human SARP3 maps to chromosome 4q31.3 and is expressed in retinal pigment epithelium (RPE) and pancreas, while expression of FRP-1,2 and 4 is high in developing tissues.
• The FRPs/SARPs are involved in the Wnt signaling pathway by regulating the intracellular levels of beta-catenin.
• Applications:Suitable for use in Immunofluorescence, Immunohistochemistry and Western Blot.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 1:500-1:1000Immunofluorescence: 1:50-1:200Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.