Forkhead box M1 (FoxM1) is a forkhead box family transcription factor that regulates a number of genes throughout the cell cycle to help control DNA replication, mitosis and cell proliferation.
FoxM1 expression increases during G1 and S and reaches maximum levels in G2/M (1-3).
Nuclear translocation occurs just before entry into G2/M and is associated with FoxM1 phosphorylation (4).
Phosphorylation of FoxM1 by MAPK (Ser331 Ser704), Cyclin/Cdk (Thr600, Thr611, Thr672), Plk1 (Ser715, Ser724), and Chk2 (Ser376) stabilizes and activates FoxM1 (4-7).
Forkhead box M1 is expressed in all embryonic tissues but is restricted to proliferating tissues in adults (8).
Recent studies have shown that FoxM1 expression is negatively regulated by p53 (9,10).
Upregulation of FoxM1 is associated with many human cancers, including prostate, breast, lung, ovary, colon, pancreas, stomach, bladder, liver, and kidney, and may be associated with p53 mutations in some tumors (10,11).
As a result, FoxM1 inhibitors have become a topic of interest for potential cancer therapy (12).
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of FOXM1. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant full length Human FOXM1.
Shelf Life：1year
Swiss Prot Number：Q08050
EU Commodity Code：30021010
商品名：FOXM1 (FKH L16, FKHL 16, FKHL16, Forkhead box M1, Forkhead box Protein M1, Forkhead drosophila Homolog like 16, Forkhead Drosophila like 16, Forkhead like 16, Forkhead Related Protein FKHL16, Forkhead-Related Protein FKHL16, FOX M1, FOXM 1, FOXM 1c, Foxm1, FOXM1_HUMAN, FOXM1B, FOXM1c, Hepatocyte Nuclear Factor 3 forkhead Homolog 11, HFH 11, HFH-11, HFH11, HNF 3, HNF 3/fork head Homolog 11, HNF 3/forkhead Homolog 11, HNF-3/fork-head Homolog 11, HNF3, Human putative M phase Phosphoprotein 2, INS 1, INS1, M phase Phosphoprotein 2, M-phase Phosphoprotein 2, MPHOSPH 2, MPHOSPH2, MPM 2, MPM 2 reactive Phosphoprotein 2, MPM-2 reactive Phosphoprotein 2, MPM2 reactive Phosphoprotein 2, MPP 2, MPP2, PIG 29, PIG29, TGT3, Transcription Factor Trident, Trident, WIN, Winged helix Factor from INS 1 Cells, Winged-helix Factor from INS-1 Cells, Winged-helix Factor from INS1 Cells,) 50ul
