84-2335-63　［取扱停止］FoxC1/2 (Forkhead box Protein C1, Forkhead-Related Protein FKHL7, Forkhead-Related Transcription Factor 3, FREAC-3, FOXC1, FKHL7, FREAC3, Forkhead box Protein C2, Forkhead-Related Protein FKHL14, Mesenchyme fork head Protein 1, MFH-1 Protein, Transcription Factor FKH-14, FOXC2, FKHL14, MFH1) 50ul　222746

特徴

• The forkhead transcription factor genes FOXC1 (Mf1) and FOXC2 (Mfh1) interact with the Notch signaling pathway and are required for the prepatterning of anterior and posterior domains in the presumptive somites through a putative Notch/Delta/Mesp regulatory loop.
• The genes have similar, dose-dependent functions, and compensate for each other in the early development of the heart, blood vessels and somites.
• Both FOXC1 and FOXC2 are expressed in the mesenchyme from which the ocular drainage structures derive.
• FOXC1 and FOXC2 also interact in kidney and heart development.
• Mutations in the FOXC1 gene result in Axenfeld-Rieger malformations of the anterior segment of the eye and lead to an increased susceptibility of glaucoma, including juvenile glaucoma.
• Functional regions in FOXC1 are required for nuclear localization and transcriptional regulation.
• Specifically, two regions in the FOXC1 forkhead domain, one rich in basic amino acid residues, and a second, highly conserved among all FOX proteins, are necessary for nuclear localization of the FOXC1 protein.
• Applications:Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.