Folate is an essential vitamin that must be obtained from food intake through intestinal absorption in mammals.
Folate and reduced folic acid derivatives bind to the folate receptor (FR) family, which mediates the endocytosis of 5-methyltetrahydrofolate into the cell.
The folate receptors consist of five members, alpha, beta, y, y' (which is produced by alternative splicing) and delta.
beta-FR is attached to the membrane by a GPI anchor and is expressed in malignant tissues of epithelial and nonepithelial origin.
y-FR is expressed in tissues of hematopoietic origin, such as spleen, thymus and bone marrow.
The expression pattern of delta-FR is elusive, which suggests that it is highly restricted both spatially and temporally.
The alpha isoform of the folate receptor is used as a highly selective tumor marker and may be targeted for the delivery of therapeutic compounds to tumor cells by coupling to derivatives of folic acid.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of FOLR3. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：A synthetic peptide corresponding to residues in Human FOLR3.
