Although the macrophage colony stimulating factor (M-CSF) and its receptor, c-Fms, are involved in the survival and proliferation of hematopoietic cells, little is known about the signalling events leading to differentiation into mature blood cells.
A 78kDa Fms-interacting protein, FMIP, transiently binds to M-CSFactivated Fms-molecules.
This binding results in a rapid phosphorylation of FMIP within its Fms-binding domain, thereby dissociating Fms and FMIP.
Endogenous levels of FMIP may form a threshold that decide whether bipotential progenitor cells differentiate into macrophages or granulocytes.
Myeloid progenitor cells express low levels of endogenous FMIP and, upon M-CSF specific signalling, are differentiated into macrophages.
Overexpression of FMIP may saturate Fms, which results in predominant cytoplasmic expression of FMIP and favors granulocyte differentiation.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of FMIP. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 570-624 of Human FMIP.
