Flt 3 ligand (Flt 3-L), variously designated Flt 3/Flk 2 ligand or FL, is a 17kDa hematopoietic growth factor that stimulates the proliferation of stem and CD34+ progenitor cells and has been cloned from both mouse and human genomes.
Flt 3-L is a potent in vitro growth stimulator of granulocytemacrophage colony-stimulating factor (GM-CSF), interleukin-3 (IL-3), and G-CSF-dependent granulocyte-macrophage committed precursors from Lin CD34+ bone marrow cells as well as other primitive B cell populations.
Additionally, Flt 3-L stimulates the proliferation of hematopoietic progenitor cells isolated from mouse fetal liver or adult mouse bone marrow.
Flt 3-L does not, however, affect the growth of erythroid-committed progenitors.
A Flt-3 ligand exists in two forms and is active as both a soluble and as a membrane-bound ligand.
The Flt 3-L receptor, Flt 3, is a tyrosine kinase expressed on CD34+ cells that shares a high degree of homology with the SCF (stem cell factor) receptor, c-Kit and c-Fms.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of Flt3-L. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 180-230 of Human Flt3-L.
