84-2335-25　［取扱停止］Flotillin-1 (FLOT 1, FLOT1, FLOT1_HUMAN, Flotillin-1, Flotillin1, Integral Membrane Component of caveolae, Reggie 2,) 50ul　222727

特徴

• Lipid rafts are sphingolipid- and cholesterol-rich membrane microdomains that are insoluble in nonionic detergents.
• Lipid rafts are important for numerous cellular processes, including signal transduction, membrane trafficking and molecular sorting.
• Flotillins are lipid raft components in neurons and caveloae-associated proteins in A498 kidney cells.
• Flotillin-1 belongs to the band 7.2/stomatin protein family, whose members are characterized by the presence of a hydrophobic N-terminal region that is predicted to form a single, outside to inside, transmembrane domain.
• Flotillin-1 and -2 have complementary tissue distributions and their expression levels are independently regulated.
• At the cellular level, Flotillin-2 is ubiquitously expressed, whereas Flotillin-1 is expressed in A498 kidney cells, muscle cell lines and fibroblasts.
• Flotillins form a ternary complex with CAP and Cbl, directing the localization of the CAP-Cbl complex to a lipid raft subdomain of the plasma membrane.
• Association of ER-X with Flotillin localizes ER-X within plasma membrane caveloae and mediates rapid oestrogen activation of the MAP kinase cascade.
• The expression of the flotillins is also correlated to the progression of Alzhemier pathology.
• Applications:Suitable for use in Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.