FOXO1A (FKHR or ForkHead in Rhabdomyosarcoma) is a 70kDa protein which is a member of a subfamily of the forkhead homeotic gene family of transcription factors.
Recent studies have shown that this protein can act as either a coactivator or a corepressor of nuclear receptor activity.
This action is mediated through the LXXLL motif found in the C terminus of the FOXO1A protein.
The specific function of the gene has not yet been determined; however, it may play a role in myogenic growth and differentiation.
Chromosomal aberrations involving FOXO1A are a cause of rhabdomyosarcoma 2 (RMS2); also known as alveolar rhabdomyosarcoma.
Applications:Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of FKHR/FoxO1, phosphorylated S256. Species Crossreactivity: Human, mouse
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic phosphopeptide derived from human FKHR around the phosphorylation site of Serine 256.
