Caldesmon, Filamin 1, nebulin and villin are differentially expressed and regulated Actin binding proteins.
Both muscular (CDh) and non-muscular (CDl) forms of caldesmon have been identified and each has been shown to bind to Actin as well as to calmodulin and Myosin.
CDh is expressed predominantly on thin filaments in smooth muscle, whereas CDl is widely expressed in nonmuscle tissues and cells.
Filamin 1, which is ubiquitously expressed and exists as a homodimer, functions to crosslink Actin to filaments.
Nebulin is a large filamentous protein specific to muscle tissue that may function as a ruler for filament length.
Several isoforms of nebulin, ranging from 700 to 900 kDa, are produced by alternative exon usage.
Villin is Ca2+-regulated and is the major structural component of the brush border of absorptive cells.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of Filamin 1. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 2100-2200 of Human Filamin 1.
