Fer is a non-receptor protein-tyrosine kinase (nRTK) of the Fes/Fps family, which shares a functional (SH2) domain and is involved in signaling pathways through receptor tyrosine kinases (RTK) and cytokine receptors.
The Fes/Fps family is distinct from c-Src, c-Abl and related nRTKs and was originally distinguished as a homolog to retroviral oncoproteins.
In vivo, Fer kinaseassembles into homotrimers via conserved coiled-coil domains.
The N-terminal coiled-coil domains of Fer can autophosphorylate in trans, thereby regulating their cellular function through differential phosphorylation states.
Growth factor exposure can induce tyrosine phosphorylation of Fer and recruitment of Fer to RTK complexes containing p85.
Fer is implicated in insulin signaling, cell-cell signaling, human prostatic proliferative diseases, and is involved in the regulation of G1 progression.
Applications:Suitable for use in Immunofluorescence, Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of Fer. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IF WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 760-810 of Human Fer.
