Muscle atrophy F-box (MAFbx) is an E3 ubiquitin ligase that initiates ATP-dependent ubiquitin-mediated proteolysis and promotes muscle atrophy.
MAFbx transcript is abundant in cardiac and skeletal muscle undergoing atrophy.
MAFbx -/- mice are resistant to muscle atrophy.
MAFbx is thought to recognize and bind to some phosphorylated proteins and promote their ubiquitination and degradation during skeletal muscle atrophy.
It interacts with MyoD by ubiquitination via a sequence found in transcriptional coactivators and therefore may play an important role in the course of muscle differentiation by determining the abundance of MyoD.
MAFbx is specifically expressed in cardiac and skeletal muscle.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of FBXO32. Species Crossreactivity: Human, mouse, rat
