The FA proteins lack sequence homologies or motifs that could point to a molecular function.
Phosphorylation of FANC(Fanconi anemia complementation group) proteins are thought to be important for the function of the FA pathway.
Several FA proteins, including FANCA, FANCC, FANCF, and FANCG, interact in a nuclear complex, and this complex is required for the activation (monoubiquitination) of the downstream FANCD2 protein.
When monoubiquitinated, the FANCD2 protein colocalizes with the breast cancer susceptibility protein BRCA1 in DNA damage induced foci.
In male meiosis, FANCD2 also colocalizes with BRCA1 at synaptonemal complexes.
The human FANCD2 gene maps to chromosome 3p25.3, contains 44 exons and encodes a 1,451 amino acid nuclear protein that exists as two protein isoforms.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of FANCD2. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 182-234 of Human FANCD2.
