84-2333-19　［取扱停止］FANCA, phosphorylated (S1149) (Fanconi anemia group A Protein, Protein FACA, FAA, FACA, FANCH) 50ul　222619

特徴

• Fanconi anemia (FA) is an autosomal recessive disorder characterized by bone marrow failure, birth defects and chromosomal instability.
• At the cellular level, FA is characterized by spontaneous chromosomal breakage and a unique hypersensitivity to DNA cross-linking agents.
• At least eight complementation groups (A-G) have been identified and six FA genes (for subtypes A, C, D2, E, F and G) have been cloned.
• The FA proteins lack sequence homologies or motifs that could point to a molecular function.
• The cellular accumulation of FA proteins, including FANCA and FANCG, is subject to regulation by TNFalpha signaling.
• Phosphorylation of FANC (Fanconi anemia complementation group) proteins is thought to be important for the function of the FA pathway.
• FANCA, also known as FACA and FANCH, associates with the Brm-related gene 1 (BRG1) product, a subunit of the SWI/SNF complex which remodels chromatin structure through a DNA-dependent ATPase activity.
• FANCA is mainly expressed in lymphoid tissues, testis and ovary.
• The amino-terminal region of the FANCA protein is required for FANCG binding, FANCC binding, nuclear localization and functional activity of the complex.
• Applications:Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Immunocytochemistry: 1:50-1:200Immunoprecipitation: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.