Ezrin, Moesin and Radixin belong to a family of highly homologous Actinassociated proteins that are localized just beneath the plasma membrane.
The proteins are believed to be involved in the mediation of interactions between cytoskeletal and membrane proteins.
Ezrin serves as a major cytoplasmic substrate of various protein-tyrosine kinases, including the epidermal growth factor receptor.
Ezrin has also been identified as a cAMP-dependent protein kinase (A-kinase) anchoring protein and designated AKAP78.
Moesin and Radixin share over 70% homology with Ezrin and are coexpressed within various cell types.
Despite the high degree of homology, the three proteins exhibit a distinct receptor-specific pattern of phosphorylation.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of Ezrinphosphorylated-T567/Radixinphosphorylated-T564/Moesinphosphorylated-T558. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic phosphopeptide derived from human Ezrin, Radixin, Moesin respectively are around the phosphorylation site of Threonine 567, 564, 558.
