ESD (esterase D) is also known as S-formylglutathione hydrolase and is a 282 amino acid protein that is a member of the esterase D family.
ESD is highly expressed in placenta, kidney, liver and erythrocytes, and is localized to the cytoplasm, as well as to cytoplasmic vesicles.
The main function of ESD is to detoxify formaldehyde while providing energy.
Formaldehyde is oxidized by ADH5 which yields S-formylglutathione.
ESD then catalyzes the hydrolysis of S-formylglutathione to the reduced forms of formic acid and glutathione.
In addition, ESD hydrolyzes a variety of different neutral ester substrates and can act as a carboxylesterase.
ESD may also act as a cysteine hydrolase which is inactivated by thiol alkylating agents.
ESD gene polymorphism can lead to reduced enzymatic activity which may cause susceptibility to many conditions, including toxic liver cirrhosis, retinoblastoma, obesity and autism.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of ESD. Species Crossreactivity: Human
