Helicase with RNase motif, more commonly designated Dicer, cleaves doublestranded RNA (dsRNA) in the RNA interference and small temporal RNA (stRNA) pathways, producing active small RNA components (siRNAs) which target the destruction of RNA and repress gene expression.
Human Dicer cleaves dsRNA independent of ATP.
The 3’-5’ exonuclease ERI-1, also known as Protein 3’hExo, degrades Histone mRNA after replication and may be involved in the regulation of RNA interference.
ERI-1 has a high affinity for the stem-loop structure of replication-dependent Histone pre-mRNAs.
It requires the 5’-ACCCA-3’ sequence present in stem-loop structure.
ERI-1 and a stemloop binding protein (SLBP) target opposite faces of a unique highly conserved stem-loop RNA scaffold towards the 3’ end of Histone mRNA.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of ERI1. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 266-318 of Human ERI1.
