ABT1 (activator of basal transcription 1) is a nuclear protein that associates with the TATA-binding protein (TBP) and enhances basal transcription activity of class II promoters.
ABT1 associates with TBP in HeLa nuclear extracts in vitro.
Another protein, designated ERF, is a member of the Ets family of transcription factors.
The members of the Ets family are grouped because they share a highly conserved DNA binding domain.
These factors are involved in growth factor pathways and regulate both proliferation and differentiation.
ERF (Ets-2 repressor factor) is a ubiquitously expressed Ets-domain protein that exhibits strong transcriptional repressor activity, suppresses Ets-induced transformation and is regulated by MAPK phosphorylation.
ERF transcription may be regulated by Ets-domain proteins.
Additionally, modulation of ERF activity is involved in the transcriptional regulation of genes activated during entry into G1 phase.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of ERF. Species Crossreactivity: Human, mouse
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to amino acids 500-550 of Human ERF.
