84-2331-29　［取扱停止］ERCC6L (ATP Dependent Helicase ERCC6 like, ATP-Dependent Helicase ERCC6-like, DNA excision Repair Protein ERCC 6 like, DNA excision Repair Protein ERCC-6-like, ERC6L_HUMAN, ERCC 6L, ERCC6L, Excision Repair cross Complementing rodent Repair deficiency complementation group 6 like, Excision Repair Protein ERCC6 like, FLJ20105, MGC131695, PICH, Plk1 interacting Check ...　222524

特徴

• PICH (Plk1-interacting checkpoint helicase), also known as DNA excision repair protein ERCC-6-like (ERCC6L) or tumor antigen BJ-HCC-15, is a 1,250 amino acid protein belonging to the SNF2/RAD54 helicase family.
• PICH is a DNA helicase and an essential component of the spindle assembly checkpoint.
• During mitosis, PICH recruits MAD2 to kinetochores and also regulates the tension on centromic chromatin.
• PICH is concentrated in between the kinetochores in prometophase cells, while in metaphase it localizes to the thin threads composed of catenated centromeric DNA that stretch between sister kinetochores.
• PICH is phosphorylated by Plk, which prevents PICH from associating with chromosome arms and restricts the localization of PICH to the kinetochore-centromere region.
• PICH/Plk interaction is also required for correct Plk localization to the kinetochore.
• PICH contains one helicase ATP-binding domain, two TPR repeats and one helicase C-terminal domain.
• Applications:Suitable for use in Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.