alpha-hemoglobin stabilizing protein (AHSP), also designated erythroid associated factor (ERAF), is an erythroid-specific protein that acts as a chaperone to prevent the aggregation of alpha-hemoglobin during normal erythroid cell development.It specifically protects free alpha-hemoglobin from precipitation in live cells and in solution.
It forms a heterodimer with free alpha-hemoglobin, but not with beta-hemoglobin or hemoglobin A (alpha2-beta-2).
AHSP localizes to the cytoplasm and is expressed in the blood and bone marrow.
The AHSP protein is downregulatedin transmissible spongiform encephalopathies (TSEs).
AHSP may regulate pathological states of alpha-hemoglobin excess such as beta-thalassemia,a group of hereditary disorders involving the decreased production of normal adult hemoglobin (HbA) that are characterized by a deficiency in the synthesis of beta-globin chains.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of ERAF. Species Crossreactivity: Human
