Erythropoietin, or Epo, is the primary factor responsible for regulating erythropoiesis during steady-state conditions and in response to blood loss and hemorrhage in the adult organism.
In addition, Epo has also been shown to play a role in primitive embryonic erythropoiesis.
Epo is synthesized by the kidney and stimulates the proliferation and maturation of bone marrow erythroid precursor cells.
Circulating Epo is a 165 amino acid glycoprotein.
The Epo receptor, EpoR, is a glycoprotein expressed on megakaryocytes, erythroid progenitors and endothelial cells.
Overexpression of Epo is associated with several pathophysiological conditions, such as polycythemias vera, which is caused by the Epo-independent growth of erythrocytic progenitors from abnormal stem cells.
A deficiency in Epo expression has been associated with afflictions such as anemia of chronic disease (ACD), frequently found in rheumatoid arthritis (RA) patients.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of EPO. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：A synthetic peptide corresponding to residues in Human EPO.
