Epoxide hydrolases (EHs) are biotransformation enzymes that catalyze the hydrolysis of arene and aliphatic epoxides to less reactive and more water soluble dihydrodiols by the trans addition of water.
The enzymatic hydration is essentially irreversible and produces mainly metabolites of lower reactivity that can be conjugated and excreted, and, therefore, are generally regarded as detoxifying.
Microsomal EH (mEH) is one of many enzymes involved in the metabolism of endogenous levels of and exogenous toxicants such as tobacco-derived carcinogens.
mEH exhibits a broad substrate specificity, while the soluble EH (sEH) is an enzyme with a "complementary" substrate specificity to mEH.
The mEH protein is encoded by the EPHX1 gene, which maps to chromosome 1q42.12.
Polymorphism of the EPHX1 gene is a risk factor ovarian cancer and hepatocellular carcinoma.
Applications:Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of EPHX1. Species Crossreactivity: Human, mouse, rat
