Ephrins, which act as ligands for Eph receptors, are cell-surface proteins which fall into two categories, ephrin-A and ephrin-B based on their structure and function.
Ephrin-B proteins are transmembrane and have conserved cytoplasmic tyrosine residues that are phosphorylated upon interaction with an EphB receptor.
Eph receptors and ephrins exhibit complementary expression in many tissues during embryogenesis indicating that bidirectional activation of Eph receptors and ephrin-B proteins may occur at expression domain interfaces.
Ephrin-B1 transduces outside-in signals through C-terminal protein interactions that effect integrin-mediated cell attachment and migration.
The distribution of ephrin-B1 in the developing retina suggests that it influences retinal axon mapping along the dorsal-ventral axis and may be involved in intratectal development.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of Ephrin-B2. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant full length Human Ephrin-B2.
