Ephrins are the ligands which bind to the Eph receptor subfamily, the largest group of the receptor tyrosine kinase family.
Both ephrins and Eph receptors are broadly expressed through the ectoderm, mesoderm, and endoderm of vertebrate embryos.
Two classes of ephrins exist: Class A ephrins are tethered to the membrane by a GPI linkage and bind primarily to EphA receptors; Class B ephrins contain a membrane-spanning region and bind predominantly to EphB receptors.
Ephrin B1 may play a role in constraining the orientation of longitudinally projecting axons.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:50ul
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity:Recognizes endogenous levels of Ephrin-B1. Species Crossreactivity: Human, mouse, rat
Isotype:IgG
Calc Applications Abbrev:IHC WB
Calc Crossreactivity:Hu Mo Rt
Immunogen:Recombinant full length Human Ephrin-B1.