The epithelial cell adhesion molecule Ep-CAM, which is also design atedtumor-associated calcium signal transducer 1 and MK-1, is a monomeric membrane glycoprotein that is expressed in most normal human epithelium and in most carcinomas.
The human Ep-CAM gene encodes a 314 amino acid protein that is expressed as two forms, a 40kDa major form and a 42kDa minor form, which are reduced to 35kDa upon treatment with the aminoglycosylation inhibitor tunicamycin.
Ep-CAM is overexpressed in a variety of carcinomas and is, therefore, a potential target for the visualization and therapy of human solid tumors.
Ep-CAM contains an extracellular domain containing two epidermal growth factor-like repeats, followed by a cysteinepoor region, which are necessary for the adhesion properties of the molecule.
Applications:Suitable for use in Immunofluorescence, Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Synthetic peptide corresponding to amino acids 120-170 of Human EPCAM.
Isotype：IgG
Calc Applications Abbrev：IF WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 120-170 of Human EPCAM.
