Prostaglandin E2, a member of the autacoid family of lipid mediators, is a major renal cyclooxygenase product of arachidonic acid metabolism.
Prostaglandin E2 binds to four G protein-coupled E-prostanoid receptors, designated EP1, EP2, EP3 and EP4.
The expression and function of the prostaglandin E2 receptors have been highly characterized in kidney.
EP1, which is predominantly expressed in the collecting duct, couples to Gq proteins to inhibit sodium absorption and increase in intracellular calcium, which act as second messengers.
EP2 is coupled to Gs proteins, which stimulate adenylyl cyclase.EP2 has the lowest expression in kidney, but EP2 knockout mice exhibit saltsensitive hypertension, which suggests a role for EP2 in salt excretion.
EP3, which is expressed in renal vessels, thick ascending limb and collecting duct, has at least six alternative splice variants that couple to Gi proteins to inhibit cAMP, which subsequently inhibit sodium and water transport.
In uterus, EP3 induces the contraction of uterine smooth muscles.
EP4 is expressed in glomerulus and collecting duct.
It couples to Gs proteins, which stimulate adenylyl cyclase and regulate glomerular tone and renal renin release.
Applications:Suitable for use in Immunofluorescence, Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of EP4. Species Crossreactivity: Human
Isotype：IgG
Calc Applications Abbrev：IF WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to amino acids 350-400 of Human EP4.
