Prostaglandin E2 binds to four G protein-coupled E-prostanoid receptors, designated EP1, EP2, EP3 and EP4.
The expression and function of the prostaglandin E2 receptors have been highly characterized in kidney.
EP3, which is expressed in renal vessels, thick ascending limb and collecting duct, has at least six alternative splice variants that couple to Gi proteins to inhibit cAMP, which subsequently inhibit sodium and water transport.
In uterus, EP3 induces the contraction of uterine smooth muscles.
EP4 is expressed in glomerulus and collecting duct.
Applications:Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of EP3. Species Crossreactivity: Human
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to amino acids N-terminus of Human EP3.
