Enolase is a glycolytic enzyme catalyzing the reaction pathway between 2 phospho glycerate and phosphoenol pyruvate.
In mammals, enolase molecules are dimers composed of three distinct subunits (alpha, beta and gamma).
The alpha subunit is expressed in most tissues and the beta subunit only in muscle.
The gamma subunit is expressed primarily in neurons, in normal and in neoplastic neuroendocrine cells.
NSE (neuron specific enolase) is found in elevated concentrations in plasma in certain neoplasias.
These include pediatric euroblastoma and small cell lung cancer.
Coexpression of NSE and chromogranin A is common in neuroendocrine neoplasms
Applications:Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of NSE/Enolase. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 361-410 of Human NSE/Enolase.
