Eukaryotic RNA polymerase II mediates the synthesis of mature and functional messenger RNA.
This is a multistep process, called the transcription cycle, that includes five stages: preinitiation, promoter, clearance, elongation and termination.
Elongation is thought to be a critical stage for the regulation of gene expression.
ELOF1 (Transcription elongation factor 1 homolog) is a 83 amino acid nuclear protein that is implicated in the maintenance of proper chromatin structure in regions that are being actively transcribed.
The gene encoding ELOF1 maps to human chromosome 19, which consists of over 63 million bases, houses ~1,400 genes and is recognized for having the greatest gene density of the human chromosomes.
It is the genetic home for a number of immunoglobulin (Ig) superfamily members, including the killer cell and leukocyte Ig-like receptors, a number of ICAMs, the CEACAM and PSG family and Fc receptors (FcRs).
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of ELOF1. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：A synthetic peptide corresponding to residues in Human ELOF1.
