84-2328-55　［取扱停止］ELAVL1 (ELAV-like Protein 1, Hu-Antigen R, HuR, ELAVL1, HUR) 100ul　222388

特徴

• The Elav-like genes encode for a family of RNA-binding proteins.
• Elav, a Drosophila protein and the first described member, is expressed immediately after neuroblastic differentiation into neurons and is necessary for neuronal differentiation and maintenance.
• Several mammalian Elav-like proteins, designated HuC, HuD and Hel-N1, are also expressed in postmitotic neurons.
• An additional mammalian homolog, HuR, which is also designated HuA, is ubiquitously expressed and is also overexpressed in a wide variety of tumors.
• Characteristically, these homologs all contain three RNA recognition motifs (RRM) and they specifically bind to AU-rich elements (ARE) in the 3'-untranslated region of mRNAs transcripts.
• ARE sites target mRNA for rapid degradation and thereby regulate the expression genes involved in cell growth and differentiation.
• When Elav-like proteins associate with these ARE sites this degradation is inhibited, leading to an increased stability of the corresponding transcript.
• Elav proteins function within the nucleus, and they are shuttled between the nucleus and cytoplasm by a nuclear export signal, which is a regulatory feature of the Elav-like proteins as it limits their accessibility to ARE sites.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.