In mammalian cells, translation is controlled at the level of polypeptide chain initiation by eukaryotic initiation factors.
The translation initiation factor 5 (eIF5) catalyzes the hydrolysis of GTP bound to the 40S ribosomal subunit, a function necessary for the subsequent joining of the 40S and 60S subunits to form the 80S initiation complex.
eIF4E specifically binds to the mRNA cap to promote unwinding and exposure of the AUG-initiation codon.
Overexpression of eIF4E can lead to cell transformation and tumorigenesis.
An additional initiation factor, eIF2, is present as a heterotrimer composed of eIF2alpha, eIF2beta and eIF2γ subunits.
This heterotrimer forms a complex with GTP and tRNA which then binds to the 40S ribosomal subunit.
After the formation of the 80S initiation complex, eIF2 is hydrolyzed and eIF2-GDP is released from the complex.
eIF2-GDP is subsequently converted to eIF2-GTP, a reaction catalyzed by eIF2B, and is then available to catalyze another round of initiation.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 500-1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of eIF5A2. Species Crossreactivity: Human, mouse
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to amino acids 96-140 of Human eIF5A2.
