84-2328-39　［取扱停止］eIF4E, phosphorylated (S209) (eIF4E ( phospho S209), eIF-4E, eIF4E, mRNA cap-Binding Protein, eIF-4F 25kD Subunit, EIF4E, 1977, EIF4E, 133440, P06730, CBP, EIF4EL1, EIF4F, MGC111573) 100ul　222380

特徴

• eIF4E, a protein modulates translation of maternal mRNAs in early embryos before the onset of zygotic transcription.
• eIF4E also influences the overall rate of translation.
• eIF4E binds to the 7 methyl GTP cap structure of eukaryotic mRNAs.
• Phosphorylation of eIF4E on serine 209 regulates the affinity of this protein for the 7 methyl GTP cap and/or RNA.
• Phosphorylation also enhances the interaction of eIF4E with eIF4G, which form a complex known as eIF4F.
• eIF4E phosphorylation is correlated with increased translational rate in a number of cell types.
• Several kinases are currently being investigated as potential regulators of eIF4E including PKC and/or the MAP kinase activated Mnk.
• Applications:Suitable for use in Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.