The initiation of protein synthesis in eukaryotic cells is regulated by interactions between protein initiation factors and RNA molecules.
The eukaryotic initiation complex eIF4F exists in vitro as a trimeric complex of eIF4G, eIF4E, and eIF4A.
Together, the complex allows ribosome binding to mRNA by inducing the unwinding of mRNA secondary structures.
eIF4E binds to the mRNA “cap” during an early step in the initiation of protein synthesis .
eIF4A acts as an ATP-dependent RNA helicase.
eIF4G acts as a bridge between eIF4E, eIF4A, and the eIF3 complex.
Applications:Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Immunocytochemistry: 1:50-1:200Immunoprecipitation: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of eIF4E. Species Crossreactivity: Human, mouse, rat
