The initiation of protein synthesis in eukaryotic cells is regulated by interactions between protein initiation factors and RNA molecules.
The eukaryotic initiation complex eIF2 exists as a heterotrimeric complex of eIF2alpha, eIF2beta and eIF2γ.
eIF2 functions in the early stages of protein synthesis, by forming a ternary complex with GTP and tRNA.
This complex binds to the 40S ribosomal subunit, followed by mRNA binding to 40S to form the 43S preinitiation complex, the release of eIF2 from 40S, and the hydrolysis of GTP.Phosphoryation of eIF2alpha correlates with inhibition of translation initiation.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of EIF2S2. Species Crossreactivity: Human, mouse, rat
