Interferon-inducible RNA-dependent protein serine/threonine kinase, PKR, is variously designated in earlier literature as DAI, dsJ, PI kinase, p65, p67 or TIK for the mouse kinase; and p68 or p69 for the human kinase.
The PKR kinase substrate is the a subunit of protein synthesis initiation factor eIF-2.
Phosphorylation of eIF-2a on serine-51 results in inhibition of translation.
Molecular cDNA clones have been isolated from both human and mouse cells.
The serine/threonine kinase catalytic domains map to the carboxy terminal half of the protein while the RNA-binding domains are located in the amino terminal region.
Three kinds of regulation of PKR enzymatic activity have been described.
These include transcriptional regulation in response to interferon, an autoregulatory mechanism controlling PKR expression at the level of translation and post-translational regulation by RNA-mediated autophosphorylation.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of Eif2ak2. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant full length Human Eif2ak2.
Shelf Life：1year
Swiss Prot Number：Q03963
EU Commodity Code：30021010
商品名：Eif2ak2 (Double stranded RNA Activated Protein Kinase,, E2AK2_HUMAN, eIF-2A Protein Kinase 2, EIF2AK1, EIF2AK2, Eukaryotic Translation Initiation Factor 2 alpha Kinase 2, Eukaryotic Translation Initiation Factor 2-alpha Kinase 2, HGNC:9437, Interferon induced double stranded RNA Activated Protein Kinase, Interferon inducible elF2 alpha Kinase, Interferon inducible RNA Dependent Protein Kinase, Interferon-induced, double-stranded RNA-Activated Protein Kinase, Interferon-inducible RNA-Dependent Protein Kinase, MGC126524, P1/eIF-2A Protein Kinase, P1/eIF2A Protein Kinase, p68 Kinase, PKR, PRKR, Protein Kinase interferon inducible double stranded RNA Dependent, Protein Kinase RNA Activated, Protein Kinase RNA-Activated, Serine/threonine Protein Kinase TIK, Tyrosine Protein Kinase EIF2AK2,) 50ul
