84-2327-32　［取扱停止］Eg5 (Kinesin-like Protein KIF11, Kinesin-like Protein 1, Kinesin-like spindle Protein HKSP, Kinesin-Related motor Protein Eg5, Thyroid Receptor-interacting Protein 5, TR-interacting Protein 5, TRIP-5, KIF11, EG5, KNSL1, TRIP5) 100ul　222325

特徴

• Eg5 (also known as Kinesin-like protein KIF11 or TRIP5) is a slow, plus-end-directed microtubule-based motor of the BimC kinesin family that is essential for bipolar spindle formation during eukaryotic cell division.
• When the expression of Eg5 is blocked, centrosome migration halts and cells are arrested in mitosis with monoastral microtubule arrays.
• Eg5 is phosphorylated on serine during S phase and on both serine and Thr 927 during mitosis, which regulates the association of Eg5 with the spindle apparatus (probably during early prophase).
• Eg5 is also known to be a member of the thyroid receptor interacting protein (TRIP) family, and interacts with the thyroid hormone receptor only in the presence of thyroid hormone.
• Applications:Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.