EFP (estrogen-responsive finger protein) is a transcription factor, the content of which is regulated by estrogen.
It has been identified as a member of the RING finger family, a family of proteins containing a Zn2+ binding domain designated the C3HC4 or RING finger.
EFP also contains two B box domains and a coiled-coil region (a transactivation domain), which are characteristic of a subgroup of the RING finger family.
Estrogen regulates the growth, differentiation and function of target cells in a variety of tissues; however, few genes have been shown to be directly regulated by estrogen.
It has been speculated that EFP may mediate estrogen activity in a signaling cascade in which estrogen-ER binding to the estrogen responsive element (ERE) downstream of the EFP gene upregulates EFP gene expression.
The EFP gene product may then activate transcription of secondary estrogen responsive genes.
Additional studies indicate that the EFP promoter may be regulated by multiple elements and their interacting factors.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of EFP. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 360-418 of Human EFP.
