Two elongation factors (EF) EF-Tu and EF-2 participate in the elongation phase during protein biosynthesis on the ribosome and their functional cycles depend on GTP binding and its hydrolysis.
EF-Tu (also designated mitochondrial precursor p43) and EF-2 are multidomain GTPases with essential functions in translation, and they both bind to the same site on the ribosome where their low intrinsic GTPase activities are strongly stimulated.
EF-Tu plays a central role in the fast and accurate delivery of aminoacyl-tRNAs to the translating ribosome.
In addition, EF-Tu protects the aminoester bond against hydrolysis until a correct match between the codon on mRNA and the anticodon on tRNA can be achieved.
EF-2 supports the translocation of tRNAs and of mRNAs on the ribosome so that a new codon can be exposed for decoding.
Applications:Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of EEF2. Species Crossreactivity: Human, mouse, rat
