The human endothelins represent a gene family comprised of endothelin-1, endothelin-2, and endothelin-3, also known as ET-1, ET-2, and ET-3.
Endothelins can affect the central nervous system and neuronal excitability, and they elicit potent vasoconstrictor action.
The two receptor subtypes responsible for inducing vasoconstriction and vasodilation, ETA and ETB, have different receptor affinities for ET-1, ET-2, and ET-3.
Of the three isopeptides, ET-2 has the most potent vasoconstrictor activity (1).
Biologically active ETs are proteolytically generated from a larger precursor, the big-endothelin, by action of the endothelin-converting enzyme (ECE) family.
ET-1 is a potent, 21-amino acid vasoconstrictor peptide produced by vascular endothelial cells.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of EDN1. Species Crossreactivity: Human, mouse, rat
