E4BP4, also known as NFIL3, functions as a transcriptional repressor and is a member of the basic leucine zipper (bZIP) transcription factor family.
E4BP4 binds with high specificity to the E4 ATF, which is a DNA sequence traditionally targeted by the ATF/CREB family of transcription factors.
A 65 amino acid segment located in the carboxy-terminus of E4BP4 interacts specifically with the TBP binding protein Dr1.
In the suprachiasmatic nucleus, circadian center and liver, E4BP4 competes with PAR proteins for DNA binding via a reciprocating mechanism.
The phase expression of E4BP4 correlates with the circadian cycle and represses transcription of genes otherwise activated by PAR transcription regulators.
E4BP4 also plays an important role in an IL-3-mediated signaling pathway that is responsible for the survival of B cell progenitors.
The gene encoding human E4BP4 maps to chromosome 9q22.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of E4BP4. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 32-86 of Human E4BP4.
