Cadherins are members of a multigene family of single chain glycoprotein receptors mediating calcium dependent cell-cell adhesion.
They play an important role in the growth and development of cells via the mechanisms of control of tissue architecture and the maintenance of tissue integrity.
Cadherins are expressed in a tissue specific manner and and are required for assembly of cells into solid tissue.
Individual cadherin molecules are known to co-operate with each other to form a linear cell adhesion zipper.
In adhesion junctions cadherins are bound to beta and gamma catenins which in turn bind to alpha catenin, an actin binding protein.
Cadherins play an important part in tumor invasion and metastasis.
Applications:Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of E-cadherin. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 840-880 of Human E-cadherin.
