Members of the Dynamin family, including Dynamin I and Dynamin II, are GTPase, microtubule-associated proteins which are involved in endocytosis, synaptic transmission and neurogenesis.
Dynamin I is localized to the central nervous system, while Dynamin II exhibits ubiquitous distribution with highest expression in testis.
Both Dynamin proteins contain SH3 and proline-rich domains that mediate interactions between the Dynamins and effectors of their GTPase activity.
The interactions with these effectors, which include microtubules, acidic phospholipids and SH3 domain-containing proteins, are required for rapid endocytosis.
Dynamin I appears to be recruited to Clathrin coated pits by SH3 domain interaction with Amphiphysin, a protein highly expressed in brain.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 15mM sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of Dynamin-1. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 750-800 of Human Dynamin-1.
