Dynactin is a multisubunit complex that functions as a binding partner for the Dynein microtubule motor.
Dynactin-Dynein binding may be required for most, if not all, cytoplasmic Dynein-driven activities and is thought to contribute to the functional diversity of Dynein.
Dynactin 2, also known as DCTN2, Dynamitin or DCTN50, is a peripheral membrane protein that is one of many subunits in the Dynactin complex.
Like other Dynactin subunits, Dynactin 2 mediates Dynein-organelle binding and helps to regulate chromosome alignment during prometaphase and spindle organization during mitosis.
Overexpression of Dynactin 2 disrupts the Dynactin-Dynein complex, thus inhibiting retrograde axonal transport and causing motor neuron degeneration.
Additionally, overexpression of Dynactin 2 may disrupt the cell cycle and lead to osteosarcoma, suggesting a possible role for Dynactin 2 in carcinogenesis
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of Dynactin 2. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant full length Human Dynactin 2 / DCTN2.
