The deduced 198 amino acid MAP kinase phosphatase 6 (MKP-6), also designated MAP6 and dual-specificity phosphatase 14 (DUSP14), is homologous to other MKP family proteins in that it has a conserved, centrally located, catalytic core, but differs from traditional MKP proteins because it contains unique N- and C-terminal regions.
Binding and deletion analyses have established that the interaction between the cytoplasmic tail of CD28 (a T cell antigen) and MKP-6 occurs at Tyr 200 of CD28 and is specific for both MKP-6 and CD28; however, Tyr 200 can be mutated to Phe 200 without a loss of binding ability.
Functional analysis indicates that MKP-6 dephosphorylates ERK, JNK and p38 while acting as a negative regulator of CD28 signaling.
MKP-6 is expressed ubiquitously, although expression is stronger in certain cell types and tissues than in others.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of DUSP14. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：A synthetic peptide corresponding to residues in Human DUSP14.
